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Astbury Centre for Structural Molecular Biology
School of Molecular and Cellular Biology
Faculty of Biological Sciences

Post-doctoral Research Fellow: Mass Spectrometrist
£30,122 – £35,938 p.a. (due to funding limitations it is anticipated that the appointment will be made at £30,122 p.a.) University Grade 7
Fixed term 2 years

Human Frontiers Science Programme – “Ribosomal signalling and specificity in co-translational protein processing and folding”

You are invited to join an international, multi-centre, interdisciplinary project funded by the Human Frontiers Science Programme. Our goal is to test the hypothesis that the ribosome plays a central role sensing the nature of the emerging nascent chain within the eukaryotic ribosomal tunnel and also that it is involved in signalling to the exterior of the ribosome to recruit the appropriate nascent polypeptide-associated factors (NAFs).

To test this hypothesis, we will investigate the conformational properties of nascent polypeptides at different stages of elongation in the presence and absence of different NAFs to map their folding properties. Specifically, you will be involved in the development and application of mass spectrometry-based techniques using ion mobility mass spectrometry, chemical modification and H/D exchange monitored by Mass Spectrometry (MS), combined with other biophysical analyses, to address these questions.

This project is between the laboratories of Professors Sheena Radford and Alison Ashcroft within the Astbury Centre for Structural Molecular Biology, in collaboration with Professors Elke Deuerling (University of Konstanz, Germany) and Judith Frydman (Stanford University, USA).

You should have, or be close to completing, a PhD in Chemistry, Biochemistry, Biophysics or a related discipline. Experience in protein mass spectrometry and non-covalent interactions in complex assemblies is essential.

For more information please see: www.astbury.leeds.ac.uk/, www.personal.leeds.ac.uk/~fbsmaspe/, www.astbury.leeds.ac.uk/bmbsgi10/serres.php

For an informal enquiry, please either contact Professor Sheena Radford at s.e.radford@leeds.ac.uk or +44 (0)113 343 3170 or Professor Alison Ashcroft at a.e.ashcroft@leeds.ac.uk or 0113 343 7273.

For more information and to apply, please click on the apply link and visit the University of Leeds website.

Closing date: 3 October 2012.